Difference between revisions of "Part:BBa K5226014:Design"

 
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===Design Notes===
 
===Design Notes===
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Due to the presence of illegal sites in this part that do not comply with RFC 10 or Type IIS standards, synonymous mutation is required for illegal sites. Mutate the base pairs at 1197bp, 1974bp and 2580bp of Vib-gcvP from g/c to a/t.
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===Source===
 
===Source===

Latest revision as of 01:42, 2 August 2024


Vib-gcvP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 546
    Illegal XbaI site found at 1602
    Illegal PstI site found at 2237
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1629
    Illegal NheI site found at 2226
    Illegal PstI site found at 2237
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 353
    Illegal BglII site found at 1638
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 546
    Illegal XbaI site found at 1602
    Illegal PstI site found at 2237
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 546
    Illegal XbaI site found at 1602
    Illegal PstI site found at 2237
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Due to the presence of illegal sites in this part that do not comply with RFC 10 or Type IIS standards, synonymous mutation is required for illegal sites. Mutate the base pairs at 1197bp, 1974bp and 2580bp of Vib-gcvP from g/c to a/t.

Source

Sequence source is from Vibrio natriegens NBRC 15636 = ATCC 14048 = DSM 759, found through NCBI （https://www.ncbi.nlm.nih.gov/nuccore/CP009978.1?from=1375714&to=1378578）

References