Difference between revisions of "Part:BBa K5226002"
 
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<partinfo>BBa_K5226002 short</partinfo>
 
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===Sequence and Features===
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<partinfo>BBa_K5226002 SequenceAndFeatures</partinfo>
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<br>
 
===Introduction===
 
===Introduction===
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One of the goals of iGEM24-SCUT-China-A is to use synthetic biology tools to obtain Halomonas TD strains that can metabolize formate. We chose to introduce the formate assimilation pathway to enable it to utilize formate, a difficult-to-recover product in CDE. For the first method, based on previous studies obtained from literature research, we selected the tetrahydrofolate (THF) cycle imported from Methylobacterium extorquens AM1(parts:) and strengthened the endogenous C2 and C3 modules of Halomonas TD(parts:).
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One of the goals of iGEM24-SCUT-China-A is to use synthetic biology tools to obtain <i>Halomonas</i> TD strains that can <b>metabolize formate</b>. We chose to <b>introduce the formate assimilation pathway</b> to enable it to utilize formate, a difficult-to-recover product in CDE. For the first method, <b>based on previous studies obtained from literature research</b>, we <b>selected the tetrahydrofolate (THF) cycle imported from <i>Methylobacterium extorquens</i> AM1 and strengthened the endogenous C2 and C3 modules of <i>Halomonas</i> TD</b>.
  
 
===Usage and Biology===
 
===Usage and Biology===
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AM1-MtdA is methylenetetrahydrofolate dehydrogenase. The protein sequence is from Methylobacterium extorquens AM1. This protein catalyzes the conversion of 5,10-methenyltetrahydrofolate into 5,10-methylene-5,6,7,8-tetrahydrofolate during the tetrahydrofolate (THF) cycle(C1M) . This part is used in BBa_xx and BBa_xx.
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AM1-MtdA is <b>methylenetetrahydrofolate dehydrogenase</b>. The protein sequence is from <i>Methylobacterium extorquens</i> AM1. This protein <b>catalyzes the conversion of 5,10-methenyltetrahydrofolate into 5,10-methylene-5,6,7,8-tetrahydrofolate during the tetrahydrofolate (THF) cycle(C1M)</b> .
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K5226002 SequenceAndFeatures</partinfo>
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Latest revision as of 09:07, 13 September 2024

AM1-mtdA

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 94
    Illegal AgeI site found at 385
  • 1000
    COMPATIBLE WITH RFC[1000]


Introduction

One of the goals of iGEM24-SCUT-China-A is to use synthetic biology tools to obtain Halomonas TD strains that can metabolize formate. We chose to introduce the formate assimilation pathway to enable it to utilize formate, a difficult-to-recover product in CDE. For the first method, based on previous studies obtained from literature research, we selected the tetrahydrofolate (THF) cycle imported from Methylobacterium extorquens AM1 and strengthened the endogenous C2 and C3 modules of Halomonas TD.

Usage and Biology

AM1-MtdA is methylenetetrahydrofolate dehydrogenase. The protein sequence is from Methylobacterium extorquens AM1. This protein catalyzes the conversion of 5,10-methenyltetrahydrofolate into 5,10-methylene-5,6,7,8-tetrahydrofolate during the tetrahydrofolate (THF) cycle(C1M) .