Difference between revisions of "Part:BBa K4451012"

Revision as of 15:53, 12 October 2023

G1 gp240

BBa_K4451008 encodes the gp240 protein from the G1 phage. This protein has been reported to interact with DnaN (DNA Pol III beta subunit) and shuts down host replication (Liu et al., 2004).

Description

An inhibitor of DNA replication from the G1 phage, which causes growth rate suppression in Staphylococcus aureus. The gp240 gene encodes a small inhibitory protein that targets and inhibits the DnaN protein in S. aureus, itself encoding a crucial subunit for DNA polymerase III. Its inhibition shows potent growth rate suppression due to the inhibition of DNA replication initiation. To date, no evidence has been shown to suggest its function in E. coli, but it has a shared function with the Coliphage N4 gene gp8, which does itself cause DNA replication arrest in E. coli (Liu et al., 2004).

Figure 5: Mean log2(OD) against time for gp240 - , concentrations refer to the [IPTG] used to induce the pLac promoter regulating gp240

As shown, there is a noticeable reduction in growth rates for various concentrations of IPTG used to induce pLac promoter regulating gp240 in comparison to the pET28 control, but the specific effects of different concentrations of IPTG cannot be determined.

Sequence and Features