Difference between revisions of "Part:BBa K4615000"
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This part will amplify the backbone of pTargetF along with targeted tpiA sgRNA sequence to generalize linear PCR fragments, products will be transformed into E.coli later and self-circulaized in T-B18. | This part will amplify the backbone of pTargetF along with targeted tpiA sgRNA sequence to generalize linear PCR fragments, products will be transformed into E.coli later and self-circulaized in T-B18. |
Latest revision as of 15:12, 12 October 2023
Forward primer to insert 20 bp of sgRNA of tpiA into pTargetF
This part will amplify the backbone of pTargetF along with targeted tpiA sgRNA sequence to generalize linear PCR fragments, products will be transformed into E.coli later and self-circulaized in T-B18.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 27
Illegal XbaI site found at 33
Illegal PstI site found at 45 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 27
Illegal PstI site found at 45 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 27
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 27
Illegal XbaI site found at 33
Illegal PstI site found at 45 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 27
Illegal XbaI site found at 33
Illegal PstI site found at 45 - 1000COMPATIBLE WITH RFC[1000]