Difference between revisions of "Part:BBa K243027"

Revision as of 22:43, 20 October 2009

His-FOS

Activator protein-1 (AP-1) is a crucial transcription factor implicated in numerous cancers. Nine homologues of the AP-1 leucine zipper region have been characterized. Via leucin zipper they interact among each other and with their bipartite domain they bind DNA. (Abate et al., Mol Cell Biol. 1991 July).

Heterodimeric Fos complex (http://www.rcsb.org/pdb/explore/images.do?structureId=1FOS)

Usage and Biology

Plasmids encoding the sequences for the bZip domains of c-Fos linked to a His-tag allow the purification which could then be expressed as soluble proteins in Escherichia coli.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 19
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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	Activator protein-1 (AP-1) is a crucial transcription factor implicated in numerous cancers. Nine homologues of the AP-1 leucine zipper region have been characterized. Via leucin zipper they interact among each other and with their bipartite domain they bind DNA. (Abate et al., Mol Cell Biol. 1991 July).		Activator protein-1 (AP-1) is a crucial transcription factor implicated in numerous cancers. Nine homologues of the AP-1 leucine zipper region have been characterized. Via leucin zipper they interact among each other and with their bipartite domain they bind DNA. (Abate et al., Mol Cell Biol. 1991 July).

−	[[Image:1fos bio r 250.jpg]] Heterodimeric Fos complex	+	[[Image:1fos bio r 250.jpg]] Heterodimeric Fos complex (http://www.rcsb.org/pdb/explore/images.do?structureId=1FOS)