Difference between revisions of "Part:BBa K4584003"

Revision as of 13:56, 12 October 2023

pSB1C3 vector with strong promoter, strong RBS, corynebacteria LldR

Create LldR expression pSB1C3 vector with strong promoter (BBa_J23100) , strong RBS (BBa_B0034) and LldR from corynebacterial (BBa_K4584000)

We designed and ordered a new composite BioBrick BBa_K4584003 with cloning linkers shown in yellow, strong promoter BioBrick BBa_J23100 (shown in magenta), strong RBS BioBrick BBa_B0034 (shown in teal) and LldR from corynebacterial BioBrick BBa_K45840000, scar sequences underlined.

The pSB1C3 vector, containing a strong promoter, strong RBS, and the corynebacterial LldR transcription factor, is crafted for expression of LldR in bacteria. This engineered plasmid allows for in detailed studies on its function and potential regulatory roles, particularly pertaining to lactate metabolism.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 15
Illegal NheI site found at 38
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 256
Illegal XhoI site found at 478
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 624
1000
COMPATIBLE WITH RFC[1000]

@@ Line 6: / Line 6: @@
 We designed and ordered a new composite BioBrick BBa_K4584003 with cloning linkers shown in yellow, strong promoter BioBrick BBa_J23100 (shown in magenta), strong RBS BioBrick BBa_B0034 (shown in teal) and LldR from corynebacterial BioBrick BBa_K45840000, scar sequences underlined.
+https://static.igem.wiki/teams/4584/wiki/screenshot-2023-10-12-at-14-01-37.png
 The pSB1C3 vector, containing a strong promoter, strong RBS, and the corynebacterial LldR transcription factor, is crafted for expression of LldR in bacteria. This engineered plasmid allows for in detailed studies on its function and potential regulatory roles, particularly pertaining to lactate metabolism.