Difference between revisions of "Part:BBa K4765130"
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===Introduction===
 
===Introduction===
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This composite part is designed to assess whether pRAP can function in cyanobacteria. It includes StayGold, stem-loop, Twister P1, and smURFP. We connect this composite part into a self-replicating shuttle vector that can replicate and function in both ''E. coli'' and cyanobacteria<ref>Chen, Y., Taton, A., Go, M., London, R. E., Pieper, L. M., Golden, S. S., & Golden, J. W. (2016). Self-replicating shuttle vectors based on pANS, a small endogenous plasmid of the unicellular cyanobacterium Synechococcus elongatus PCC 7942. ''Microbiology'', ''162''(12), 2029–2041. https://doi.org/10.1099/mic.0.000377</ref>..
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This composite part is designed to assess whether pRAP can function in cyanobacteria. It includes stayGold, stem-loop, Twister P1, and smURFP. We connect this composite part into a self-replicating shuttle vector that can replicate and function in both ''E. coli'' and cyanobacteria<ref>Chen, Y., Taton, A., Go, M., London, R. E., Pieper, L. M., Golden, S. S., & Golden, J. W. (2016). Self-replicating shuttle vectors based on pANS, a small endogenous plasmid of the unicellular cyanobacterium Synechococcus elongatus PCC 7942. ''Microbiology'', ''162''(12), 2029–2041. https://doi.org/10.1099/mic.0.000377</ref>.
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When both red and green fluorescence can be expressed, it can be concluded that pRAP is functional in cyanobacteria. This provides a convenient expression system for genetic modification in cyanobacteria.
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When both green and far-red fluorescence can be detected, we conclude that pRAP is functional in cyanobacteria. This provides a convenient expression system for genetic modification in cyanobacteria, which itself fluorescence in red.
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===Characterization===
 
===Characterization===

Revision as of 15:34, 12 October 2023


shuttle test contributed by Fudan iGEM 2023


Introduction

This composite part is designed to assess whether pRAP can function in cyanobacteria. It includes stayGold, stem-loop, Twister P1, and smURFP. We connect this composite part into a self-replicating shuttle vector that can replicate and function in both E. coli and cyanobacteria[1]. When both green and far-red fluorescence can be detected, we conclude that pRAP is functional in cyanobacteria. This provides a convenient expression system for genetic modification in cyanobacteria, which itself fluorescence in red.

Characterization

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1181
    Illegal BsaI.rc site found at 1201


  1. ↑ Chen, Y., Taton, A., Go, M., London, R. E., Pieper, L. M., Golden, S. S., & Golden, J. W. (2016). Self-replicating shuttle vectors based on pANS, a small endogenous plasmid of the unicellular cyanobacterium Synechococcus elongatus PCC 7942. Microbiology, 162(12), 2029–2041. https://doi.org/10.1099/mic.0.000377