Difference between revisions of "Part:BBa K4765127"

(→‎Characterization)
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===Introduction===
 
===Introduction===
−
Similar to [https://parts.igem.org/Part:BBa_K4765126 BBa_K4765126(ribozyme connected: ''H. ex'' mtSSB + AnAFP + SAHS 33020)], we changed the order of ''H. ex'' mtSSB, SAHS 33020 and AnAFP.
+
Similar to [https://parts.igem.org/Part:BBa_K4765126 BBa_K4765126], we changed the order of ''H. ex'' mtSSB, SAHS 33020 and AnAFP.
 +
 
 
===Characterization===
 
===Characterization===
 
Get details in [https://parts.igem.org/Part:BBa_K4765126 BBa_K4765126].
 
Get details in [https://parts.igem.org/Part:BBa_K4765126 BBa_K4765126].

Revision as of 13:00, 12 October 2023


ribozyme connected: H. ex mtSSB + AnAFP + SAHS 33020 contributed by Fudan iGEM 2023

Introduction

Similar to BBa_K4765126, we changed the order of H. ex mtSSB, SAHS 33020 and AnAFP.

Characterization

Get details in BBa_K4765126.

Agarose gel electrophoresis

contributed by Fudan iGEM 2023
Figure 1. Agarose gel electrophoresis of PCR products, amplified from bacterial colonies/cultures.

From left lane(1) to right lane(3) indicate the successful construction of H. ex mtSSB, H. ex mtSSB + AnAFP, and H. ex mtSSB + AnAFP + SAHS 33020.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 247
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1573
    Illegal BsaI.rc site found at 515
    Illegal BsaI.rc site found at 596
    Illegal SapI site found at 409
    Illegal SapI.rc site found at 1214