Difference between revisions of "Part:BBa K225001:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | ScaS/EnvZ fusion was designed by combining the light receptor domain of ScaS ( | + | ScaS/EnvZ fusion was designed by combining the light receptor domain of ScaS (chromatic acclimation sensor from Synechocystis sp PCC 6803) with EnvZ. |
− | + | The histidine kinase domain in ScaS was changed to EnvZ. | |
− | + | ||
− | + | ||
===Source=== | ===Source=== |
Latest revision as of 21:34, 20 October 2009
Green light activated actuator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 2202
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1564
Illegal BglII site found at 2456 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1117
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
ScaS/EnvZ fusion was designed by combining the light receptor domain of ScaS (chromatic acclimation sensor from Synechocystis sp PCC 6803) with EnvZ. The histidine kinase domain in ScaS was changed to EnvZ.
Source
ScaS from Synechocysitis sp. PCC 6803
References
Hirose et al. (2008) Cyanobacteriochrome CcaS is the green light receptor that induces the expression of phycobilisome linker protein. Proc Natl Acad Sci U S A.105(28), 9528-33