Difference between revisions of "Part:BBa K4800019"
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Revision as of 12:51, 12 October 2023


EutM-SpyCatcher

We constructed EutM-SpyCatcher building blocks and attached itself covalently via isopeptide bond formation by the SpyTag-SpyCatcher system to the formed scaffolds，joining with enzyme elements to form a complex and improving the stability of the enzyme element. <p> Sequence and Features

Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 42
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 42
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 42
    Illegal BamHI site found at 36
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 42
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 42
  • 1000
    COMPATIBLE WITH RFC[1000]