Difference between revisions of "Part:BBa K4683010:Experience"

Revision as of 13:47, 12 October 2023

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K4683010

Lambert_GA 2023
We characterized the padlock and the miRNA sequence, but unfortunately, we were unable to characterize these parts due to lack of time.

User Reviews

UNIQ405a99b329b1d1f7-partinfo-00000000-QINU UNIQ405a99b329b1d1f7-partinfo-00000001-QINU

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 <b> Lambert_GA 2023</b>
 <br>
-Gel Experimentation
+We characterized the padlock and the miRNA sequence, but unfortunately, we were unable to characterize these parts due to lack of time.
-<br>
-We performed RCA on these six miRNAs (miR-20b, miR-328,miR-146, miR-150, miR-122, miR-30c) and ran the subsequent rolling circle product (RCP) on a 1% agarose gel (see Fig.). The gel exhibited a fluorescent band of DNA very close to the well, indicating that a long strand of DNA greater than 1 kB - our RCP - was produced. Therefore, we can validate that our reaction was successful.
-<br>
-<html><img src="https://static.igem.wiki/teams/4683/wiki/rca-optimization/screenshot-2023-10-11-at-4-49-47-pm.png"
-alt="Figure 1. Image of gel ran with miRNA-1 RCP product; A: eRCA with 40.8 pM miR-1; B: negative control (no enzymes)
-" width="500"></html>
-<br>
-Figure. RCP from RCA reaction with miR-20b, miR-328,miR-146, miR-150, miR-122, and miR-30c; visible bands can be seen near the top of the wells.
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