Difference between revisions of "Part:BBa K4645008:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | OmpA is added to the N terminus of the ClyR protein. | + | OmpA is added to the N-terminus of the ClyR protein. |
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| − | + | ||
===Source=== | ===Source=== | ||
Revision as of 14:08, 12 October 2023
A chimeric lysozyme with secretory ability.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 816
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 184
Illegal AgeI site found at 268
Illegal AgeI site found at 583
Illegal AgeI site found at 766 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
OmpA is added to the N-terminus of the ClyR protein.
Source
The catalytic domain come from Streptococcus virus CI and the cell-wall binding domain come from Streptococcus suis.
References
[1] Xu J, Yang H, Bi Y, Li W, Wei H, Li Y. Activity of the Chimeric Lysin ClyR against Common Gram-Positive Oral Microbes and Its Anticaries Efficacy in Rat Models. Viruses. 2018 Jul 20;10(7):380. [2] Yang H, Linden SB, Wang J, Yu J, Nelson DC, Wei H. A chimeolysin with extended-spectrum streptococcal host range found by an induced lysis-based rapid screening method. Sci Rep. 2015 Nov 26;5:17257.