Difference between revisions of "Part:BBa K4683009:Experience"
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<b> Lambert_GA 2023</b>
 
<b> Lambert_GA 2023</b>
 
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Gel Experimentation
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Unfortunately, we were not able to characterize it.
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We performed RCA on these six miRNAs (miR-20b, miR-328,miR-146, miR-150, miR-122, miR-30c) and ran the subsequent rolling circle product (RCP) on a 1% agarose gel (see Fig.). The gel exhibited a fluorescent band of DNA very close to the well, indicating that a long strand of DNA greater than 1 kB - our RCP - was produced. Therefore, we can validate that our reaction was successful.
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<html><img src="https://static.igem.wiki/teams/4683/wiki/rca-optimization/screenshot-2023-10-11-at-4-49-47-pm.png"
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alt="Figure 1. Image of gel ran with miRNA-1 RCP product; A: eRCA with 40.8 pM miR-1; B: negative control (no enzymes)
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Figure. RCP from RCA reaction with miR-20b, miR-328,miR-146, miR-150, miR-122, and miR-30c; visible bands can be seen near the top of the wells.
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<I>Username</I>
 
<I>Username</I>
 
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Enter the review information here.
 
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Revision as of 13:44, 12 October 2023


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K4683009

Lambert_GA 2023
Unfortunately, we were not able to characterize it.



User Reviews

UNIQ79c95723bb62346d-partinfo-00000000-QINU UNIQ79c95723bb62346d-partinfo-00000001-QINU