Difference between revisions of "Part:BBa K4584003"

 
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TTTACGGCTAGCTCAGTCCTAGGTACAATGCTAGCTACTAGAGAAAGAGGAGAAATACTAGatgagtgtgaaagcacatgaatctgtcatggattgggtcaccgaggaactccgcagcggtcgcctaaaaatcggtgatcatctccccagcgaacgggcgctctccgaaaccctcggagtttcccgaagctccctgcgcgaggcgcttcgtgtgctcgaagccctcggcaccatttccaccgccaccggatccggaccgcggtctggcaccatcatcaccgctgcccctggccaggcgctttccctctccgtgacgctgcaattggtcaccaaccaggtcggccaccacgatatttatgaaacccgccaactccttgaaggctgggctgccctgcattccagcgccgaacgcggcgactgggacgtggcagaagcgttgctggaaaagatggatgaccccacgctaccgctcgaggattttttgcgtttcgacgccgagttccacgtcgttatctccaaaggcgcggaaaaccctctgatcagtacgctcatggaagccctccgcttgtccgtggcagatcacaccgttgccagggctctggcgctcccagattggccggcgacctcagctcgcctccagaaagaacaccgtgcaatcctcgcagcacttcgcgcaggtgaatccaccatggcagcaaccctgatcaaagaccacatcgaaggctattaccaagaaaccgctgctgccgaggcataa
 
  
 
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Revision as of 12:32, 12 October 2023


pSB1C3 vector with strong promoter, strong RBS, corynebacteria LldR

Create LldR expression pSB1C3 vector with strong promoter (BBa_J23100) , strong RBS (BBa_B0034) and LldR from corynebacterial (BBa_K4584000)

We designed and ordered a new composite BioBrick BBa_K4584003 with cloning linkers shown in yellow, strong promoter BioBrick BBa_J23100 (shown in magenta), strong RBS BioBrick BBa_B0034 (shown in teal) and LldR from corynebacterial BioBrick BBa_K45840000, scar sequences underlined.

bba-k4584003-33-map.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 15
    Illegal NheI site found at 38
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 256
    Illegal XhoI site found at 478
  • 23
    INCOMPATIBLE WITH RFC[23]
    Unknown
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 624
  • 1000
    COMPATIBLE WITH RFC[1000]