Difference between revisions of "Part:BBa K4451010"
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A transcriptional inhibitor from T4 phage, which causes growth rate suppression in E. coli. | A transcriptional inhibitor from T4 phage, which causes growth rate suppression in E. coli. | ||
The AsiA gene encodes an anti-σ factor, which is able to bind to σ70 and prevent the RNA polymerase σ70 holoenzyme from recognising σ70 specific promoters, thus enacting a transcriptional downregulation of housekeeping genes and subsequently causing growth rate suppression. AsiA further enables the transcription of phage middle genes as part of the T4 infection cycle (J. Liu et al., 2004). | The AsiA gene encodes an anti-σ factor, which is able to bind to σ70 and prevent the RNA polymerase σ70 holoenzyme from recognising σ70 specific promoters, thus enacting a transcriptional downregulation of housekeeping genes and subsequently causing growth rate suppression. AsiA further enables the transcription of phage middle genes as part of the T4 infection cycle (J. Liu et al., 2004). | ||
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| + | https://static.igem.wiki/teams/4939/wiki/assets/assets/registry-assets/bba-k4451010-meanlog2od.png | ||
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| + | Figure 1: Mean log2(OD) against time for AsiA | ||
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Revision as of 14:22, 12 October 2023
T4 AsiA
BBa_K4451010 encodes the AsiA protein from the T4 phage. This protein has been reported to interact with sigma-70 factor and inhibits recognition of sigma-70 promoters (Ouhammouch et al., 1995).
Description
A transcriptional inhibitor from T4 phage, which causes growth rate suppression in E. coli. The AsiA gene encodes an anti-σ factor, which is able to bind to σ70 and prevent the RNA polymerase σ70 holoenzyme from recognising σ70 specific promoters, thus enacting a transcriptional downregulation of housekeeping genes and subsequently causing growth rate suppression. AsiA further enables the transcription of phage middle genes as part of the T4 infection cycle (J. Liu et al., 2004).
Figure 1: Mean log2(OD) against time for AsiA
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]