Difference between revisions of "Part:BBa K190019:Experience"

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The capacity of fMT to enhance the arsenite accumulation in ''E. coli'' was tested using an arsenite uptake [[http://2009.igem.org/Team:Groningen/Protocols assay]]. But unfortunately the arsenic concentration determined by ICP-MS was not reproducible and did not show enhanced arsenite accumulation in ''E. coli'' carrying fMT (induced by lactose).
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The capacity of fMT to enhance the arsenite accumulation in ''E. coli'' was tested using an arsenite uptake [http://2009.igem.org/Team:Groningen/Protocols assay]. But unfortunately the arsenic concentration determined by ICP-MS was not reproducible and did not show enhanced arsenite accumulation in ''E. coli'' carrying fMT (induced by lactose).
 
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Revision as of 19:48, 20 October 2009

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K190019

fMT can be used to sequester metal ions like arsenite (As-III) or arsenate (As-V), this can be used to give the bacterium a higher resistance against arsenic. But the protein can also be used as a delay system together with a metal sensitive promoter (as BBa_K190023), as it sequesters the arsenic as soon as it enters the cell. Upon overexpression of the protein it would take some time before it is saturated and free arsenic can reach the promoter regulator protein (like ArsR). Alternatively the protein can be used to use bacteria for biomining of arsenic, as the presence of rare metals will also become less prominent, like the fossil oil problem the earth just has a maximum amount of some compounds.

User Reviews

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iGEM Groningen 2009

The capacity of fMT to enhance the arsenite accumulation in E. coli was tested using an arsenite uptake [http://2009.igem.org/Team:Groningen/Protocols assay]. But unfortunately the arsenic concentration determined by ICP-MS was not reproducible and did not show enhanced arsenite accumulation in E. coli carrying fMT (induced by lactose).

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