Difference between revisions of "Part:BBa K4584002"
| Line 6: | Line 6: | ||
https://static.igem.wiki/teams/4584/wiki/bba-k4584002-map.png | https://static.igem.wiki/teams/4584/wiki/bba-k4584002-map.png | ||
| + | |||
| + | We designed and ordered a new composite BioBrick BBa_K4584002 with cloning linkers shown in yellow, medium promoter BioBrick BBa_J23110 (shown in magenta), LldR operator region BioBrick BBa_K4584001 (shown in grey), strong RBS BioBrick BBa_B0034 (shown in teal) and GFP BioBrick BBa_E0040 (shown in green), scar sequences underlined. | ||
| + | https://static.igem.wiki/teams/4584/wiki/sequence-composite-pt-1.png | ||
Medium Promoter | Medium Promoter | ||
Revision as of 12:36, 12 October 2023
New composite part medium promoter, operator and strong RBS (+GFP)
New composite part Medium promoter BBa_J23110, LldR operator BBa_K4584001, strong RBS B0034 and GFP BBa_E0040
We designed and ordered a new composite BioBrick BBa_K4584002 with cloning linkers shown in yellow, medium promoter BioBrick BBa_J23110 (shown in magenta), LldR operator region BioBrick BBa_K4584001 (shown in grey), strong RBS BioBrick BBa_B0034 (shown in teal) and GFP BioBrick BBa_E0040 (shown in green), scar sequences underlined.
Medium Promoter https://parts.igem.org/Part:BBa_J23110 LldR operator https://parts.igem.org/Part:BBa_K4584001 Strong RBS https://parts.igem.org/Part:BBa_B0034 GFP https://parts.igem.org/Part:BBa_E0040
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 724