Difference between revisions of "Part:BBa K4601207:Design"
 
Line 9: Line 9:
  
 
===Source===
 
===Source===
 +
 +
DNA synthesis, PCR amplifications and Golden Gate assembly
  
 
===References===
 
===References===

Latest revision as of 13:47, 12 October 2023


Jaws-NpHTRII-EnvZ expression cassette in the Evolution.T7 mutational region


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 880
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1115
    Illegal AgeI site found at 2229
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1256


Design Notes

Source

DNA synthesis, PCR amplifications and Golden Gate assembly

References