Difference between revisions of "Part:BBa K243010:Design"
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The cloning steps were planed theoretically before we started the work in the wet lab. | The cloning steps were planed theoretically before we started the work in the wet lab. | ||
The combiantion of HisTag linked with a FluA Tag connect by the Split Linker approved as a good way to express the inactive protein domain[https://parts.igem.org/wiki/index.php?title=Part:BBa_K243001] of our universal endonuclease. | The combiantion of HisTag linked with a FluA Tag connect by the Split Linker approved as a good way to express the inactive protein domain[https://parts.igem.org/wiki/index.php?title=Part:BBa_K243001] of our universal endonuclease. | ||
| − | The parts are fused with [https://parts.igem.org/Assembly_standard_25 RFC 25]. | + | The parts are fused with [https://parts.igem.org/Assembly_standard_25 RFC 25].<br> |
[https://static.igem.org/mediawiki/parts/6/6a/HisFluASplitFoki.txt Commented GenBank file] | [https://static.igem.org/mediawiki/parts/6/6a/HisFluASplitFoki.txt Commented GenBank file] | ||
Revision as of 19:22, 20 October 2009
His-FluA-Split Linker-Fok_i
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 272
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The cloning steps were planed theoretically before we started the work in the wet lab.
The combiantion of HisTag linked with a FluA Tag connect by the Split Linker approved as a good way to express the inactive protein domain[1] of our universal endonuclease.
The parts are fused with RFC 25.
Commented GenBank file
Source
Combined the parts by serial cloning steps.