Difference between revisions of "Part:BBa K4770029"
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Level Ms are the assembly of different transcriptional units into the same vector. | Level Ms are the assembly of different transcriptional units into the same vector. | ||
All AlgaGenix’s Level M follow the same structure: they contain the HygroR cassette, level 1 for each gene (pCM1:NR, NiR, GS, Cp-GS, IPT or LOG), mVenus cassette, and mCherry cassette. | All AlgaGenix’s Level M follow the same structure: they contain the HygroR cassette, level 1 for each gene (pCM1:NR, NiR, GS, Cp-GS, IPT or LOG), mVenus cassette, and mCherry cassette. | ||
| − | In this case, we are using pCM1: | + | In this case, we are using pCM1:Cp_GS |
| − | With this vector, we aim to see the effects of overexpressing | + | With this vector, we aim to see the effects of overexpressing GS constitutively in the chloroplast and at the same time normalizing the expression level depending on the insertion site of the vector (by measuring the signal of the mCherry cassette), at the same time, we will be able to see the decrease of nitrates concentration fue to higher assimilation thanks to the mVenus cassette signal. |
===How can this part be used=== | ===How can this part be used=== | ||
| − | This Level M is aimed to assembled with HygroR cassette in position 4, | + | This Level M is aimed to assembled with HygroR cassette in position 4, Cp-GS Level1 in position 5, mCherry cassette in position 6 and mVenus cassette in position 7. |
Should you use this part and need advice on how to use it, please do not hesitate to contact us at algagenix@gmail.com | Should you use this part and need advice on how to use it, please do not hesitate to contact us at algagenix@gmail.com | ||
===Source of this part=== | ===Source of this part=== | ||
Position 4: Hygro R cassette BBa_K4770016 | Position 4: Hygro R cassette BBa_K4770016 | ||
| − | Position 5: pCM1 | + | Position 5: pCM1 Cp-GS BBa_K4770021 |
Position 6: mCherry cassette BBa_K4770017 | Position 6: mCherry cassette BBa_K4770017 | ||
Position 7: mVenus cassette BBa_K4770025 | Position 7: mVenus cassette BBa_K4770025 | ||
===Design considerations=== | ===Design considerations=== | ||
AlgaGenix’s Level Ms are designed to have the same structure and not having to build different pieces with different positions for each transcriptional unit. Each piece has it’s own design considerations that can be looked up on the individual part’s pages. | AlgaGenix’s Level Ms are designed to have the same structure and not having to build different pieces with different positions for each transcriptional unit. Each piece has it’s own design considerations that can be looked up on the individual part’s pages. | ||
| + | |||
<partinfo>BBa_K4770029 SequenceAndFeatures</partinfo> | <partinfo>BBa_K4770029 SequenceAndFeatures</partinfo> | ||
Revision as of 11:26, 12 October 2023
Cp_GS_Chloroplast_Glutamate_Synthetase_Level_M
Contents
Description and Biology
Level Ms are the assembly of different transcriptional units into the same vector. All AlgaGenix’s Level M follow the same structure: they contain the HygroR cassette, level 1 for each gene (pCM1:NR, NiR, GS, Cp-GS, IPT or LOG), mVenus cassette, and mCherry cassette. In this case, we are using pCM1:Cp_GS With this vector, we aim to see the effects of overexpressing GS constitutively in the chloroplast and at the same time normalizing the expression level depending on the insertion site of the vector (by measuring the signal of the mCherry cassette), at the same time, we will be able to see the decrease of nitrates concentration fue to higher assimilation thanks to the mVenus cassette signal.
How can this part be used
This Level M is aimed to assembled with HygroR cassette in position 4, Cp-GS Level1 in position 5, mCherry cassette in position 6 and mVenus cassette in position 7. Should you use this part and need advice on how to use it, please do not hesitate to contact us at algagenix@gmail.com
Source of this part
Position 4: Hygro R cassette BBa_K4770016 Position 5: pCM1 Cp-GS BBa_K4770021 Position 6: mCherry cassette BBa_K4770017 Position 7: mVenus cassette BBa_K4770025
Design considerations
AlgaGenix’s Level Ms are designed to have the same structure and not having to build different pieces with different positions for each transcriptional unit. Each piece has it’s own design considerations that can be looked up on the individual part’s pages.
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 4830
Illegal PstI site found at 7168
Illegal PstI site found at 8686 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 4830
Illegal PstI site found at 7168
Illegal PstI site found at 8686
Illegal NotI site found at 8422 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 4830
Illegal BglII site found at 5168
Illegal BamHI site found at 4474
Illegal XhoI site found at 4686 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 4830
Illegal PstI site found at 7168
Illegal PstI site found at 8686 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 4830
Illegal PstI site found at 7168
Illegal PstI site found at 8686
Illegal NgoMIV site found at 1265
Illegal NgoMIV site found at 1447
Illegal NgoMIV site found at 4032
Illegal NgoMIV site found at 4984
Illegal NgoMIV site found at 9163 - 1000COMPATIBLE WITH RFC[1000]