Difference between revisions of "Part:BBa K4591013"
| Line 2: | Line 2: | ||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K4591013 short</partinfo> | <partinfo>BBa_K4591013 short</partinfo> | ||
| − | |||
| − | |||
===Usage and Biology=== | ===Usage and Biology=== | ||
<p>This circuit contains the coding sequences of P43, DEPO-PETase, and MHETase. The expression of enzyme genes related to this route is controlled by the frequently expressed promoter P43, which degrades PET into TPA and achieves the preliminary degradation of PET.</p> | <p>This circuit contains the coding sequences of P43, DEPO-PETase, and MHETase. The expression of enzyme genes related to this route is controlled by the frequently expressed promoter P43, which degrades PET into TPA and achieves the preliminary degradation of PET.</p> | ||
Revision as of 11:02, 12 October 2023
T7-DepoPETase-T500
Usage and Biology
This circuit contains the coding sequences of P43, DEPO-PETase, and MHETase. The expression of enzyme genes related to this route is controlled by the frequently expressed promoter P43, which degrades PET into TPA and achieves the preliminary degradation of PET.
Basic components of the circuit
P43：Promoter 43 is a constitutive promoter that constitutively expresses the P43 protein in B.subtilis.
DepoPETase：DepoPETase is the best mutant obtained by researchers using a novel high-throughput screening method based on fluorescence detection for directed evolution of IsPETase.
MHETase：MHETase is discovered from Ideonella sakaiensis 201-F6, it is a enzyme which can hydrolyze MHET, which is the main product of PETase when hydrolyzing PET.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 469
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 239
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 25