Difference between revisions of "Part:BBa K4694002"
| Line 4: | Line 4: | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
The enzyme QQ7 was proposed to be a quorum quenching enzyme which therefore inhibits biofilm formation of pathogens such as <i>Candida albicans</i> and <i>Staphylococcus epidermidis</i> [1]. The DNA sequence was identified during a metagenomic screen of bacteria that quench biofilm formation. | The enzyme QQ7 was proposed to be a quorum quenching enzyme which therefore inhibits biofilm formation of pathogens such as <i>Candida albicans</i> and <i>Staphylococcus epidermidis</i> [1]. The DNA sequence was identified during a metagenomic screen of bacteria that quench biofilm formation. | ||
| − | |||
| − | |||
| − | |||
| + | This sequence is taken from GenBank JX870909 but mutated to remove forbidden restriction sites and with the addition of prefix and suffix sequences compatible with TypeIIS cloning. The sequence was synthesised by IDT. | ||
Revision as of 11:00, 12 October 2023
His-QQ-7
Usage and Biology
The enzyme QQ7 was proposed to be a quorum quenching enzyme which therefore inhibits biofilm formation of pathogens such as Candida albicans and Staphylococcus epidermidis [1]. The DNA sequence was identified during a metagenomic screen of bacteria that quench biofilm formation.
This sequence is taken from GenBank JX870909 but mutated to remove forbidden restriction sites and with the addition of prefix and suffix sequences compatible with TypeIIS cloning. The sequence was synthesised by IDT.
Characterisation
References
[1] Weiland-Brauer, N., Malek, I. & Schmitz, R. A. 2019. Metagenomic quorum quenching enzymes affect biofilm formation of Candida albicans and Staphylococcus epidermidis. PLoS One, 14, e0211366.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]