Difference between revisions of "Part:BBa K265011"

Revision as of 18:03, 21 October 2009

OmpA with proteolytic cleavage tag

Since OmpA is believed to function similarly to INPNC and INPNC believed to function better when it is used with the right signal sequence, we have decided to test and see if OmpA's ability to secret increases when it is used by a signal sequence. We have submitted this part to the part registry. For more information go to: BBa_K265011

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K265011 short</partinfo>
-This part is a fusion of a modified OmpA with a signal sequence to allow cleavage when a protein is fused. OmpA was modified by replacing the normal restriction sites with sites for RFC12 fusion format.
+Since OmpA is believed to function similarly to INPNC and INPNC believed to function better when it is used with the right signal sequence, we have decided to test and see if OmpA's ability to secret increases when it is used by a signal sequence.
-An OmpA/protein fusion normally allows display of proteins on the cell surface by way of a linker. The addition of this signal sequence allows secretion by cleavage from the OmpA linker after display.
+We have submitted this part to the part registry.
+For more information go to: BBa_K265011
 <!-- Add more about the biology of this part here