Difference between revisions of "Part:BBa K265008"
Line 2: Line 2:
 
<partinfo>BBa_K265008 short</partinfo>
 
<partinfo>BBa_K265008 short</partinfo>
  
−
Ice nucleation protein sequence.  This exogenous gene was found to be express proteins on the surface of E. coli.  This part can be used to secrete target proteins instead of going through Sec, SRP or TAT pathways.  Because of size limitations on folded proteins, this may be a favorable device over other secretion pathways. The NC stands for abbreviated version of the entire gene which works better in E. coli.    
+
The ice-nucleation protein (INP) from Pseudomonas syringae,    is used by its natural host to nucleate ice formation and is implicated in P. syringae associated pathogenesis.  INP and a truncated derivative lacking the central domain (INPNC) have been used extensively for displaying proteins on the surface of E. coli (7,9).  For instance, PROTEINS X and Y have been successfully displayed on the surface of E. coli using INPNC (REFS). 
 +
 
 +
Park et al. have shown that INPNC when fused to the phaZ1 gene, including its signal sequence, can serve as a suitable surface delivery and secretion device of the otherwise toxic phaZ1 gene product (REF new INPNC-SS paper).
 +
 
 +
This part was synthesized by Mr. Gene (city, country) with codon optimization and subsequently transferred into vector (part name for AK vector).  As it is expected that this part will be used in the context of the fusion protein, the prefix and suffix for this part are consistent with the BBF RCF-12 standard.
 +
 
 +
——————————————————————————————————————
 +
 
 +
We have proposed to build and test a general protein secretion system modeled after that developed by Park et al. in which a fusion of INPNC and the signal sequence from the phaZ1 gene are used to secrete any target protein.  
 +
 
 +
 
 +
We have modified this protein to be consistent with BBF RFC-12 Standard. We have submitted this part to the parts registry as part BBa_K265008.  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 17:57, 21 October 2009

Ice Nucleation Protein NC

The ice-nucleation protein (INP) from Pseudomonas syringae, is used by its natural host to nucleate ice formation and is implicated in P. syringae associated pathogenesis. INP and a truncated derivative lacking the central domain (INPNC) have been used extensively for displaying proteins on the surface of E. coli (7,9). For instance, PROTEINS X and Y have been successfully displayed on the surface of E. coli using INPNC (REFS).

Park et al. have shown that INPNC when fused to the phaZ1 gene, including its signal sequence, can serve as a suitable surface delivery and secretion device of the otherwise toxic phaZ1 gene product (REF new INPNC-SS paper).

This part was synthesized by Mr. Gene (city, country) with codon optimization and subsequently transferred into vector (part name for AK vector). As it is expected that this part will be used in the context of the fusion protein, the prefix and suffix for this part are consistent with the BBF RCF-12 standard.

——————————————————————————————————————

We have proposed to build and test a general protein secretion system modeled after that developed by Park et al. in which a fusion of INPNC and the signal sequence from the phaZ1 gene are used to secrete any target protein.


We have modified this protein to be consistent with BBF RFC-12 Standard. We have submitted this part to the parts registry as part BBa_K265008.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 405
  • 1000
    COMPATIBLE WITH RFC[1000]