Difference between revisions of "Part:BBa K265008:Design"

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===Design Notes===
 
===Design Notes===
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INPNC was put in RFC12 format to accommodate fusion proteins.
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INPNC was put in RFC12 format to accommodate fusion proteins.  INPNC is anchored to the cell surface by a glycosyl-phosphatidylinositol (GPI) anchor.  The full length ice-nucleation protein is made of 3 parts--the N-terminal, the C-terminal, and a central domain.  In this truncated version (INPNC), just the N-terminal and C-terminal domains are included, indicated by the 'NC' in INPNC.  Both the full and truncated versions of INP will display fused proteins on the cell surface although transporting the protein across the inner membrane may be improved with the addition of a proteolytic cleavage sequence such as BBa_K265002.
  
 
===Source===
 
===Source===

Revision as of 18:15, 20 October 2009

Ice Nucleation Protein NC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 405
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

INPNC was put in RFC12 format to accommodate fusion proteins. INPNC is anchored to the cell surface by a glycosyl-phosphatidylinositol (GPI) anchor. The full length ice-nucleation protein is made of 3 parts--the N-terminal, the C-terminal, and a central domain. In this truncated version (INPNC), just the N-terminal and C-terminal domains are included, indicated by the 'NC' in INPNC. Both the full and truncated versions of INP will display fused proteins on the cell surface although transporting the protein across the inner membrane may be improved with the addition of a proteolytic cleavage sequence such as BBa_K265002.

Source

Synthesized from Mr. Gene. Gene originates from Pseudomonas syringae

References