Difference between revisions of "Part:BBa K4814012"

Latest revision as of 09:57, 12 October 2023

Strong RBS

To improve the RecA (K3) - B0034 - EGFP bioreporter we designed to test RecA(K3) (BBa_K3020001) promoter, we replaced the RBS with B0032 and a new RBS invented by Zhang, M. et al (2022). The researchers developed a machine learning model to predict the translation initiation rate of different RBS sequences. Then, the designed sequences were synthesized and experimentally tested for their translation initiation rates. However, our results indicated that Strong RBS did not improve performance of the reporter. Sequence: TTTAAGAGGGGGCTATACAT

Click here for the experiment data: RecA(K3)-Strong-eGFP BBa_K4814013

References:

Zhang, M., Holowko, M. B., Zumpe, H. H., & Ong, C. S. (2022). Machine Learning Guided Batched Design of a Bacterial Ribosome Binding Site. ACS Synthetic Biology, 11(7), 2314-2326. https://doi.org/10.1021/acssynbio.2c00015

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4814012 short</partinfo>
-To improve the RecA (K3) - B0034 - EGFP bioreporter we designed to test RecA(K3) (BBa_K3020001) promoter, we replaced the RBS with B0032 and a new RBS invented by Zhang, M. et al (2022). The researchers developed a machine learning model to predict the translation initiation rate of different RBS sequences. Then, the designed sequences were synthesized and experimentally tested for their translation initiation rates.
+To improve the RecA (K3) - B0034 - EGFP bioreporter we designed to test RecA(K3) (BBa_K3020001) promoter, we replaced the RBS with B0032 and a new RBS invented by Zhang, M. et al (2022). The researchers developed a machine learning model to predict the translation initiation rate of different RBS sequences. Then, the designed sequences were synthesized and experimentally tested for their translation initiation rates. However, our results indicated that Strong RBS did not improve performance of the reporter.
 Sequence: TTTAAGAGGGGGCTATACAT