Difference between revisions of "Part:BBa K212006:Design"

 
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<partinfo>BBa_K212006 short</partinfo>
 
<partinfo>BBa_K212006 short</partinfo>
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===Design Notes===
 
===Design Notes===
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blah fill this in
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Designed using Rosetta 3 macromolecular modeling program.
  
  
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===Source===
 
===Source===
  
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synthesized incorporating 16 mutations from native e. coli RBP
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synthesized DNA incorporating 16 mutations from native E. coli RBP gene.
  
 
===References===
 
===References===
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1. Looger et al. Computational design of a receptor and sensor proteins with novel functions. Nature, 2003.
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2. Mowbray et al. 1.7 Å X-ray structure of the periplasmic ribose receptor from Escherichia coli. J. Mol. Bio., 1992.

Revision as of 00:18, 22 October 2009

mRBP (modified ribose binding protein - now binds histamine)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Designed using Rosetta 3 macromolecular modeling program.


Source

synthesized DNA incorporating 16 mutations from native E. coli RBP gene.

References

1. Looger et al. Computational design of a receptor and sensor proteins with novel functions. Nature, 2003. 2. Mowbray et al. 1.7 Å X-ray structure of the periplasmic ribose receptor from Escherichia coli. J. Mol. Bio., 1992.