Difference between revisions of "Part:BBa K4825004:Design"
| Line 14: | Line 14: | ||
===Source=== | ===Source=== | ||
| − | Bacillus thuringiensis | + | <i>Bacillus thuringiensis</i> |
===References=== | ===References=== | ||
孙明, 郭素霞, 王鹏霞, 彭东海, 阮丽芳, 喻子牛. Nematicidal bacillus thuringiensis crystal protein gene cry1518-35. WO2010078708A1 [P]. 2010-07-15. | 孙明, 郭素霞, 王鹏霞, 彭东海, 阮丽芳, 喻子牛. Nematicidal bacillus thuringiensis crystal protein gene cry1518-35. WO2010078708A1 [P]. 2010-07-15. | ||
Latest revision as of 11:49, 12 October 2023
Cry1518-35
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 132
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
"1. Codons are optimized for E.coli based when it is expressed in E.coli, and optimized for S.cerevisiae based when it is expressed in S.cerevisiae. 2. 6x His tag (HHHHHH) is added to either the N-terminal or C-terminal of the coding sequence for Ni-NTA purification."
Source
Bacillus thuringiensis
References
孙明, 郭素霞, 王鹏霞, 彭东海, 阮丽芳, 喻子牛. Nematicidal bacillus thuringiensis crystal protein gene cry1518-35. WO2010078708A1 [P]. 2010-07-15.