Difference between revisions of "Part:BBa K4825003:Design"
| Line 14: | Line 14: | ||
===Source=== | ===Source=== | ||
| − | Pinus taeda | + | <i>Pinus taeda</i> |
===References=== | ===References=== | ||
CHEN Tianhua, ZHANG Ruosi, JIANG Guozhen, YAO Mingdong, LIU Hong, WANG Ying, XIAO Wenhai, YUAN Yingjin. Metabolic engineering of Saccharomyces cerevisiae for pinene production[J]. CIESC Journal, 2019, 70(1): 179-188 | CHEN Tianhua, ZHANG Ruosi, JIANG Guozhen, YAO Mingdong, LIU Hong, WANG Ying, XIAO Wenhai, YUAN Yingjin. Metabolic engineering of Saccharomyces cerevisiae for pinene production[J]. CIESC Journal, 2019, 70(1): 179-188 | ||
Latest revision as of 11:48, 12 October 2023
PS
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 223
Illegal BamHI site found at 1060 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 695
Design Notes
"1. The 51-position prolinate site at the N terminal of PtPS was truncated to increase the efficiency of pinene production. 2. PS is linked to ERG20ww with a flexible linker (GGGGSGGGGS) to increase the production of pinene from GPP, and reduce the GPP allocated to produce FPP to increase the yield of target product."
Source
Pinus taeda
References
CHEN Tianhua, ZHANG Ruosi, JIANG Guozhen, YAO Mingdong, LIU Hong, WANG Ying, XIAO Wenhai, YUAN Yingjin. Metabolic engineering of Saccharomyces cerevisiae for pinene production[J]. CIESC Journal, 2019, 70(1): 179-188