Difference between revisions of "Part:BBa K4825002:Design"
| Line 13: | Line 13: | ||
===Source=== | ===Source=== | ||
| − | Saccharomyces cerevisiae | + | <i>Saccharomyces cerevisiae</i> |
===References=== | ===References=== | ||
CHEN Tianhua, ZHANG Ruosi, JIANG Guozhen, YAO Mingdong, LIU Hong, WANG Ying, XIAO Wenhai, YUAN Yingjin. Metabolic engineering of Saccharomyces cerevisiae for pinene production[J]. CIESC Journal, 2019, 70(1): 179-188 | CHEN Tianhua, ZHANG Ruosi, JIANG Guozhen, YAO Mingdong, LIU Hong, WANG Ying, XIAO Wenhai, YUAN Yingjin. Metabolic engineering of Saccharomyces cerevisiae for pinene production[J]. CIESC Journal, 2019, 70(1): 179-188 | ||
Latest revision as of 11:46, 12 October 2023
ERG20ww
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 222
Illegal BamHI site found at 1059 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 694
Design Notes
Geranyl pyrophosphate(GPP) is precursor of pinene, which is our desired product. Therefore a higher yield of GPP is desirable, while the basic level of FPP is crucial for yeast survival. A method is designed to increase production of GPP while keeping FPP at a low level. It is to introduce 2 mutation F96W and N127W in ERG20, creating ERG20ww. The mutation in ERG20ww enables the reduction in FPP synthesis(from GPP), while GPP synthesis is not influenced. As a result, less GPP will be transformed to FPP, and a higher yield of pinene could be achieved.
Source
Saccharomyces cerevisiae
References
CHEN Tianhua, ZHANG Ruosi, JIANG Guozhen, YAO Mingdong, LIU Hong, WANG Ying, XIAO Wenhai, YUAN Yingjin. Metabolic engineering of Saccharomyces cerevisiae for pinene production[J]. CIESC Journal, 2019, 70(1): 179-188