Difference between revisions of "Part:BBa K4759206"
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we fused six protein tags (SUMO, GST, MBP, TF, Trx, Nus) at the N-terminal of the Olep. Protein tags MBP and TF can significantly improve the solubility of Olep, but the catalytic efficiency of Olep was reduced. | we fused six protein tags (SUMO, GST, MBP, TF, Trx, Nus) at the N-terminal of the Olep. Protein tags MBP and TF can significantly improve the solubility of Olep, but the catalytic efficiency of Olep was reduced. | ||
Revision as of 07:38, 12 October 2023
T7-RBS4-TF-linker-OleP
we fused six protein tags (SUMO, GST, MBP, TF, Trx, Nus) at the N-terminal of the Olep. Protein tags MBP and TF can significantly improve the solubility of Olep, but the catalytic efficiency of Olep was reduced.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 591
Illegal AgeI site found at 1428
Illegal AgeI site found at 1526 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 159
Illegal SapI.rc site found at 807
Illegal SapI.rc site found at 920
Illegal SapI.rc site found at 1176