Difference between revisions of "Part:BBa K4765136"
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====Agarose gel electrophoresis==== | ====Agarose gel electrophoresis==== | ||
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| − | | <html><img style="width:200px" src="https://static.igem.wiki/teams/4765/wiki/zsl/dna-gel/ | + | | <html><img style="width:200px" src="https://static.igem.wiki/teams/4765/wiki/zsl/dna-gel/ssb-tdp-xrcc1-dsup.png" alt="contributed by Fudan iGEM 2023"></html> |
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| '''Figure 1. Agarose gel electrophoresis of PCR products, amplified from bacterial colonies/cultures. | | '''Figure 1. Agarose gel electrophoresis of PCR products, amplified from bacterial colonies/cultures. | ||
Revision as of 07:02, 12 October 2023
ribozyme connected: FEN1 + H. ex mtSSB + SAHS 33020 + XRCC1 + Dsup
Usage and Biology
This part is the combination of all the anti-desiccation and anti-UV proteins, we've transformed it to E .coli BL21 DE3 and test its performance.
Characterization
Agarose gel electrophoresis
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| Figure 1. Agarose gel electrophoresis of PCR products, amplified from bacterial colonies/cultures.
From right lane(3) to left lane(1) indicate the successful construction of FEN1 + H. ex mtSSB, FEN1 + H. ex mtSSB + SAHS 33020, and FEN1 + H. ex mtSSB + SAHS 33020 + XRCC1 . |
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 3304
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 617
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1195
Illegal NgoMIV site found at 1513
Illegal AgeI site found at 301
Illegal AgeI site found at 5095
Illegal AgeI site found at 5166 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2146
Illegal BsaI.rc site found at 1781
Illegal BsaI.rc site found at 1862
Illegal BsaI.rc site found at 4354
Illegal BsaI.rc site found at 5092
Illegal SapI site found at 1675
Illegal SapI.rc site found at 5821