Difference between revisions of "Part:BBa K4765127"
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====Agarose gel electrophoresis==== | ====Agarose gel electrophoresis==== | ||
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| − | | <html><img style="width: | + | | <html><img style="width:200px" src="https://static.igem.wiki/teams/4765/wiki/zsl/dna-gel/ssb-anafp-tdp.png" alt="contributed by Fudan iGEM 2023"></html> |
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| '''Figure 1. Agarose gel electrophoresis of PCR products, amplified from bacterial colonies/cultures. | | '''Figure 1. Agarose gel electrophoresis of PCR products, amplified from bacterial colonies/cultures. | ||
Revision as of 06:40, 12 October 2023
ribozyme connected: H. ex mtSSB + AnAFP + SAHS 33020
Introduction
Similar to BBa_K4765126(ribozyme connected: H. ex mtSSB + AnAFP + SAHS 33020), we changed the order of H. ex mtSSB, SAHS 33020 and AnAFP.
Characterization
Get details in BBa_K4765126(ribozyme connected: H. ex mtSSB + AnAFP + SAHS 33020).
Agarose gel electrophoresis
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| Figure 1. Agarose gel electrophoresis of PCR products, amplified from bacterial colonies/cultures.
From left lane(1) to right lane(3) indicate the successful construction of H. ex mtSSB, H. ex mtSSB + AnAFP, and H. ex mtSSB + AnAFP + SAHS 33020. |
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 247
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1573
Illegal BsaI.rc site found at 515
Illegal BsaI.rc site found at 596
Illegal SapI site found at 409
Illegal SapI.rc site found at 1214