Difference between revisions of "Part:BBa K4815014:Experience"
| Line 1: | Line 1: | ||
| − | |||
__NOTOC__ | __NOTOC__ | ||
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
how you used this part and how it worked out. | how you used this part and how it worked out. | ||
| − | + | ===Applications of BBa_K4815013=== | |
| − | ===Applications of | + | We used a synthetic promoter to drive the expression of the YeGFP gene on the same plasmid, while the TEF1 promoter was used in the reverse orientation to drive the expression of the mCherry gene. Additionally, we incorporated a lactose-inducible switch to enhance safety. We utilized flow cytometry to monitor the two fluorescence signals excited by different light channels and analyzed the corresponding data. We plotted the natural logarithm of the ratio of GFP to mCherry (ln(GFP/mCherry)) as a frequency distribution graph to showcase the relative expression strength of different promoters in yeast. The figure bellow shows success in utilizing the plasmids in detecting fluorescence intensity. |
| + | <html> | ||
| + | <figure><center> | ||
| + | <img alt="" | ||
| + | src="https://static.igem.wiki/teams/4815/wiki/result/7.png" | ||
| + | width="400" | ||
| + | title=""> | ||
| + | </figure> | ||
| + | </html> | ||
===User Reviews=== | ===User Reviews=== | ||
| − | <!-- DON'T DELETE --><partinfo> | + | <!-- DON'T DELETE --><partinfo>BBa_K4815013 StartReviews</partinfo> |
<!-- Template for a user review | <!-- Template for a user review | ||
{|width='80%' style='border:1px solid gray' | {|width='80%' style='border:1px solid gray' | ||
|- | |- | ||
|width='10%'| | |width='10%'| | ||
| − | <partinfo> | + | <partinfo>BBa_K4815013 AddReview number</partinfo> |
<I>Username</I> | <I>Username</I> | ||
|width='60%' valign='top'| | |width='60%' valign='top'| | ||
| Line 18: | Line 25: | ||
|}; | |}; | ||
<!-- End of the user review template --> | <!-- End of the user review template --> | ||
| − | <!-- DON'T DELETE --><partinfo> | + | <!-- DON'T DELETE --><partinfo>BBa_K4815013 EndReviews</partinfo> |
Revision as of 06:25, 12 October 2023
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K4815013
We used a synthetic promoter to drive the expression of the YeGFP gene on the same plasmid, while the TEF1 promoter was used in the reverse orientation to drive the expression of the mCherry gene. Additionally, we incorporated a lactose-inducible switch to enhance safety. We utilized flow cytometry to monitor the two fluorescence signals excited by different light channels and analyzed the corresponding data. We plotted the natural logarithm of the ratio of GFP to mCherry (ln(GFP/mCherry)) as a frequency distribution graph to showcase the relative expression strength of different promoters in yeast. The figure bellow shows success in utilizing the plasmids in detecting fluorescence intensity.
User Reviews
UNIQ36551111e6a3a9dd-partinfo-00000001-QINU UNIQ36551111e6a3a9dd-partinfo-00000002-QINU