Difference between revisions of "Part:BBa K216009:Experience"
(→Applications of BBa_K216009) |
(→User Reviews) |
||
| Line 8: | Line 8: | ||
===User Reviews=== | ===User Reviews=== | ||
| + | |||
| + | '''Initial Tests: Edinburgh iGEM 2009:''' To test this part, we grew ''E. coli'' JM109 overnight at 37 C with shaking in LB with 80 mg/l ampicillin and varying concentrations of sodium nitrate and sodium nitrite. The following day, we performed Miller assays (LacZ activity assays using o-nitrophenyl galactoside, ONPG, as a chromogenic substrate). The results showed strong induction by both nitrate and nitrite, as expected for this promoter (see biology information for part BBa_K216005). | ||
| + | |||
<!-- DON'T DELETE --><partinfo>BBa_K216009 StartReviews</partinfo> | <!-- DON'T DELETE --><partinfo>BBa_K216009 StartReviews</partinfo> | ||
<!-- Template for a user review | <!-- Template for a user review | ||
Revision as of 16:29, 20 October 2009
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K216009
This part was designed to characterize the activity of the PyeaR promoter by Miller assay, as a backup to the GFP-based construct used in the promoter characterization kit.
User Reviews
Initial Tests: Edinburgh iGEM 2009: To test this part, we grew E. coli JM109 overnight at 37 C with shaking in LB with 80 mg/l ampicillin and varying concentrations of sodium nitrate and sodium nitrite. The following day, we performed Miller assays (LacZ activity assays using o-nitrophenyl galactoside, ONPG, as a chromogenic substrate). The results showed strong induction by both nitrate and nitrite, as expected for this promoter (see biology information for part BBa_K216005).
UNIQ396dc85ab34cdf33-partinfo-00000000-QINU UNIQ396dc85ab34cdf33-partinfo-00000001-QINU