Difference between revisions of "Part:BBa K4960013"

Revision as of 03:37, 12 October 2023 (view source) Palpitate (Talk | contribs) ← Older edit		Latest revision as of 14:08, 12 October 2023 (view source) CirceTerry (Talk | contribs) (→‎Profile)
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	Name: pvc14<br>		Name: pvc14<br>
	Base Pairs: 1716bp<br>		Base Pairs: 1716bp<br>
−	Origin: Photorhabdus<br>	+	Origin: <i>Photorhabdus</i><br>
	Properties: Pvc14 possibly controls tube optimal length<br>		Properties: Pvc14 possibly controls tube optimal length<br>
	<!-- Add more about the biology of this part here-->		<!-- Add more about the biology of this part here-->
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	===Usage and Biology===		===Usage and Biology===
	Photorhabdus virulence cassette (PVC) is one group of extracellular Contractile injection systems (CISs) that are cell-puncturing nanodevices for targeted delivery of proteins .[1]Structurally similar to other CISs, PVCs are also macromolecular complexes containing a rigid tube structure housed in a contractile sheath, which is anchored to a baseplate and sharpened by a spike protein.[2]('''Figure1''')<br>		Photorhabdus virulence cassette (PVC) is one group of extracellular Contractile injection systems (CISs) that are cell-puncturing nanodevices for targeted delivery of proteins .[1]Structurally similar to other CISs, PVCs are also macromolecular complexes containing a rigid tube structure housed in a contractile sheath, which is anchored to a baseplate and sharpened by a spike protein.[2]('''Figure1''')<br>

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Latest revision as of 14:08, 12 October 2023

pvc14
Pvc14 possibly controls tube optimal length.

Profile

Name: pvc14
Base Pairs: 1716bp
Origin: Photorhabdus
Properties: Pvc14 possibly controls tube optimal length

Usage and Biology

Photorhabdus virulence cassette (PVC) is one group of extracellular Contractile injection systems (CISs) that are cell-puncturing nanodevices for targeted delivery of proteins .[1]Structurally similar to other CISs, PVCs are also macromolecular complexes containing a rigid tube structure housed in a contractile sheath, which is anchored to a baseplate and sharpened by a spike protein.[2](Figure1)

Figure 1. Schematic of the PVCpnf locus. It contains 16 structural and accessory genes , followed by two payload genes (Pdp1 and Pnf, in red) and four putative regulatory genes (in pink).

Pvc14 is a homolog of Afp14 in Serratia Afp, which was proposed to act as a tape measure protein.According to previous studies,it is present at very low levels in the PVC syringes sample.Significantly,its deletion resulted in a large variation in the length of PVC particles.Therefore,it possibly controls tube optimal length.

Sequence and Features

References

[1]Kreitz J, Friedrich MJ, Guru A, Lash B, Saito M, Macrae RK, Zhang F. Programmable protein delivery with a bacterial contractile injection system. Nature. 2023 Apr;616(7956):357-364. doi: 10.1038/s41586-023-05870-7. Epub 2023 Mar 29.
[2]Jiang F, Li N, Wang X, Cheng J, Huang Y, Yang Y, Yang J, Cai B, Wang YP, Jin Q, Gao N. Cryo-EM Structure and Assembly of an Extracellular Contractile Injection System. Cell. 2019 Apr 4;177(2):370-383.e15. doi: 10.1016/j.cell.2019.02.020. Epub 2019 Mar 21.