Difference between revisions of "Part:BBa K4788004:Design"

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===Design Notes===
 
===Design Notes===
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it codes FadR, it is a transcription factor that can bind to the FadBA promoter, inhibit initiation, and be released by oleoyl CoA metabolized from oleic acid.
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It is a basic operator component that can combine with the FadR gene and FadBA promoter gene to form oleic acid inducers, exerting rich functions. And changing its position and quantity can change the induction threshold of the oleic acid inducer.
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===Source===
 
===Source===

Latest revision as of 01:56, 12 October 2023


FadO operator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It is a basic operator component that can combine with the FadR gene and FadBA promoter gene to form oleic acid inducers, exerting rich functions. And changing its position and quantity can change the induction threshold of the oleic acid inducer.

Source

We synthesize this gene.

References

Ma Y, Zheng X, Lin Y, Zhang L, Yuan Y, Wang H, Winterburn J, Wu F, Wu Q, Ye JW, Chen GQ. Engineering an oleic acid-induced system for Halomonas, E. coli and Pseudomonas. Metab Eng. 2022 Jul;72:325-336. doi: 10.1016/j.ymben.2022.04.003. Epub 2022 May 2. PMID: 35513297.