Difference between revisions of "Part:BBa K283013"

Latest revision as of 11:59, 21 October 2009

prosseA-rbs

The prosseA promoter with a strong rbs. The prosseA promoter comes from pathogenic bacteria Samonella 7207 strain. It is the Salmonella pathogenicity island II regulator. SsrB is autoregulated and activates expression of SPI-2 and SPI-2 co-regulated genes in response to an unknown signal(s).SsrB is autoregulated and activates expression of SPI-2 and SPI-2 co-regulated genes in response to an unknown signal(s). The preliminary data in our lab shows that the activation this regulator depends on the presence of SsrB protein without SsrA on the chromosome of E.coli and salmonella (single copy). However, when we use this prosseA based on the plasmid (not single copy) it can trigger high gene expression level. This part does not pose any biological threat.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 372
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 114
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K283013 short</partinfo>
-The prosseA promoter with a strong rbs. The prosseA promoter comes from pathogenic bacteria ''Samonella'' 7207 strain. It is the ''Salmonella'' pathogenicity island II regulator. SsrB is autoregulated and activates expression of SPI-2 and SPI-2 co-regulated genes in response to an unknown signal(s).SsrB is autoregulated and activates expression of SPI-2 and SPI-2 co-regulated genes in response to an unknown signal(s).  The preliminary data in our lab shows that the activation this regulator depends on the presence of SsrB protein without SsrA on the chromosome of E.coli and salmonella (single copy). However, when we use this prosseA based on the plasmid (not single copy) it can trigger high gene expression level.
+The prosseA promoter with a strong rbs. The prosseA promoter comes from pathogenic bacteria ''Samonella'' 7207 strain. It is the ''Salmonella'' pathogenicity island II regulator. SsrB is autoregulated and activates expression of SPI-2 and SPI-2 co-regulated genes in response to an unknown signal(s).SsrB is autoregulated and activates expression of SPI-2 and SPI-2 co-regulated genes in response to an unknown signal(s).  The preliminary data in our lab shows that the activation this regulator depends on the presence of SsrB protein without SsrA on the chromosome of E.coli and salmonella (single copy). However, when we use this prosseA based on the plasmid (not single copy) it can trigger high gene expression level. This part does not pose any biological threat.
 <!-- Add more about the biology of this part here