Difference between revisions of "Part:BBa K4788003"
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It can express BCoAT,butyryl-CoA: acetate CoA-transferase,which is involved in the synthesis of butyrate. Its expression can be utilized to synthesize butyric acid.
 
It can express BCoAT,butyryl-CoA: acetate CoA-transferase,which is involved in the synthesis of butyrate. Its expression can be utilized to synthesize butyric acid.
  
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===Usage and Biology===
 
===Usage and Biology===
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It can serve as a gene for producing short chain fatty acids and plays an important role in the production of butyric acid by microorganisms.
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===Characterization===
 
===Characterization===
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We use gas chromatography to detect the production of short chain fatty acids by strains containing the BCoAT gene in the culture medium, in order to characterize the ability of the BCoAT gene.
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As shown in Figure 1，the experimental results indicate that the BCoAT gene indeed has the ability to produce short chain fatty acids, especially the production of butyric acid, which shows significant differences compared to the two control groups.
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<figure style="text-align:center;">
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                <img style="max-width:700px;" src="https://static.igem.wiki/teams/4788/wiki/proof/bcoat-single-gene.jpg" alt="control">
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                <figcaption><b>Figure1:</b> GC detection of acetic acid, propionic acid, and butyric acid production in BCoAT single gene strains</figcaption>
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              </figure>
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</html>
  
  

Revision as of 01:48, 12 October 2023


BCoAT

It can express BCoAT,butyryl-CoA: acetate CoA-transferase,which is involved in the synthesis of butyrate. Its expression can be utilized to synthesize butyric acid.

Usage and Biology

It can serve as a gene for producing short chain fatty acids and plays an important role in the production of butyric acid by microorganisms.

Characterization

We use gas chromatography to detect the production of short chain fatty acids by strains containing the BCoAT gene in the culture medium, in order to characterize the ability of the BCoAT gene. As shown in Figure 1，the experimental results indicate that the BCoAT gene indeed has the ability to produce short chain fatty acids, especially the production of butyric acid, which shows significant differences compared to the two control groups.

control
Figure1: GC detection of acetic acid, propionic acid, and butyric acid production in BCoAT single gene strains


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 997
  • 1000
    COMPATIBLE WITH RFC[1000]