Difference between revisions of "Part:BBa K283003"
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<partinfo>BBa_K283003 short</partinfo>
 
<partinfo>BBa_K283003 short</partinfo>
  
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This part was designed to generate the cheZ proteins which are responsible for the dephosphorylation of cheY protein involved in bacteria flagella movement. A 6-histidine tail is added to the cheZ encoding region. The pTet can be repressed by the TetR repressor protein(C0040). It is constitutively on without the presence of TetR repressor. The backbone of this biobrick is based on BBa_J61003. CheZ_his tag and strong double terminators lie between XbaI and SpeI.  
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This device is an improved version of BBa_K094102. It was designed to express the CheZ, which is responsible for the dephosphorylation of CheY involved in bacterial flagella rotation. Since lacking an antibody to recognize the expressed CheZ, we fused a His6-tag to the ''cheZ'' encoding region. We put'' cheZ'' gene under the control of the pTet promoter, which is repressed by the TetR repressor protein(C0040) and can be induced by adding anhydrotetracycline (aTc). The backbone of this biobrick is BBa_J61003. The strong double terminators lie between ''Xba''I and ''Spe''I sites.  
  
  

Revision as of 09:11, 21 October 2009

pTet-rbs-chez_histag-double terminator

This device is an improved version of BBa_K094102. It was designed to express the CheZ, which is responsible for the dephosphorylation of CheY involved in bacterial flagella rotation. Since lacking an antibody to recognize the expressed CheZ, we fused a His6-tag to the cheZ encoding region. We put cheZ gene under the control of the pTet promoter, which is repressed by the TetR repressor protein(C0040) and can be induced by adding anhydrotetracycline (aTc). The backbone of this biobrick is BBa_J61003. The strong double terminators lie between XbaI and SpeI sites.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 726
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]