Difference between revisions of "Part:BBa K3002017:Experience"

Revision as of 00:01, 12 October 2023

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Applications of BBa_K3002017

User Reviews

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 <!-- Template for a user review
-<p>We detected the expression of the POR with HA-tag via immunoblotting.</p>
+===User Reviews===
-<p>
+<!-- DON'T DELETE --><partinfo>BBa_K3002006 StartReviews</partinfo>
-  <img class="bild" src="https://static.igem.wiki/teams/4806/wiki/registry/level2/por-ha-wb.png">
+<!-- Template for a user review
-  <div class="unterschrift"><b>Fig.2 Expression of the POR with HA-tag</b><br>
+{|width='80%' style='border:1px solid gray'
-   (a)Level 2 MoClo construct for expression of the POR containing the HA-tag was designed (see Fig.1 for part description) <br> (b) Picture of resulting western blot. The POR is marked by a black arrow, the white arrow marks a cross reaction of antibodies. For reference, the UVM4 recipient strain and a strain expressing the HA-tagged ribosomal chloroplast 50S protein L5 (RPL5) were used as a negative and positive control, respectively
+|-
- </div>
+|width='10%'|
-</p>
+<partinfo>BBa_K3002006 AddReview number</partinfo>
-<p>For detection the UVM4 strain was transformed with the construct in (a). 30 hygromycin-resistant transformants were cultivated in TAP medium and samples were taken after 3 days. Whole-cell proteins were extracted and analyzed by SDS-PAGE and immunoblotting using an anti-HA antibody. The expression of the POR (~ 77 kDa) is visible.
+<I>Username</I>
-</p>
+|width='60%' valign='top'|
-;
+Enter the review inofrmation here.
+|};
 <!-- End of the user review template -->
 <!-- DON'T DELETE --><partinfo>BBa_K3002017 EndReviews</partinfo>