Difference between revisions of "Part:BBa K283002"

Revision as of 07:53, 21 October 2009

Speed control device (pLacI-rbs-TetR-terminator-ptet-rbs-chez_his-double terminators)

To test if cheZ expression level was proportional to the bacterial swimming speed and hopefuly to control the speed, we designed and made this speed controller device. The full length cheZ gene fused with His6-tag was put under the control of pTet, whose expression is repressed by constitutively expressed TetR and can be induced by the addition of anhydrotetracycline or tetracycline. Since lacking antibody to recognize the CheZ protein, His6-tag was added to facilitate the measurement. This device has been demonstrated to work in our system (see experience or visit our wiki).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1782
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K283002 short</partinfo>
-The speed control device was designed to control the gene expression of cheZ_his tag by using anhydrotetracycline or tetracycline.In the presence of anhydrotetracycline or tetracycline, which binds to TetR and remove its inhibiting effect on pTet promoter, the chez-histag(BBa_K283008) protein can be expressed. Consequently, the expression level of chez-histag,which responsible for the dephosphorylation of cheY protein in bacteria flagella movement, should be controlled by the concentration of anhydrotetracycline or tetracycline.
+To test if ''cheZ'' expression level was proportional to the bacterial swimming speed and hopefuly to control the speed, we designed and made this speed controller device. The full length ''cheZ'' gene fused with His6-tag was put under the control of pTet, whose expression is repressed by constitutively expressed TetR and can be induced by the addition of anhydrotetracycline or tetracycline. Since lacking antibody to recognize the CheZ protein, His6-tag was added to facilitate the measurement. This device has been demonstrated to work in our system (see experience or visit our wiki).
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