Difference between revisions of "Part:BBa K283000"
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<partinfo>BBa_K283000 short</partinfo>
 
<partinfo>BBa_K283000 short</partinfo>
  
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This polar expression device was used for polar expression of streptavidin with his tag on the polar of our ''salmonella'' strain YBS01.  
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Streptavidin protein binds strongly to the biotin molecule, and is widely used to bind biotinylated materials. This device was designed for polar expression of streptavidin on the outer membrane of bacterial cells. To facilitate the observation, the fusion protein of Lpp signal peptide, OmpA aa46-66, and streptavidin wild-type (BBa_J36848) was fused with GFP-coding sequence (BBa_e0040). The expression will display streptavidin-GFP fusion protein on the cell surface.  
  
  

Revision as of 07:14, 21 October 2009

pTet-rbs-lpp_ompA_eGFP_streptavidin-double Terminators

Streptavidin protein binds strongly to the biotin molecule, and is widely used to bind biotinylated materials. This device was designed for polar expression of streptavidin on the outer membrane of bacterial cells. To facilitate the observation, the fusion protein of Lpp signal peptide, OmpA aa46-66, and streptavidin wild-type (BBa_J36848) was fused with GFP-coding sequence (BBa_e0040). The expression will display streptavidin-GFP fusion protein on the cell surface.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1272
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1314
    Illegal AgeI site found at 1365
  • 1000
    COMPATIBLE WITH RFC[1000]