Difference between revisions of "Part:BBa K4604006"
 
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This BioBrick is a mutated Version of BioBrick <a href="https://parts.igem.org/Part:BBa_K4604005">BBa_K4604005</a>, the gene for the BluB enzyme that catalyzes the reaction of FMNH2 to DMB used in the AdoCbl synthesis pathway in <i>E. coli</i>. Ultimately leads to AdoCbl (B12) synthesis in cultures supplemented with cobinamide. Also used in <a href="https://parts.igem.org/Part:BBa_K4604020">BBa_K4604020</a> , a more detailed characterization is uploaded there. </html>
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This BioBrick is a mutated Version of BioBrick <a href="https://parts.igem.org/Part:BBa_K4604005">BBa_K4604005</a>, the gene for the BluB enzyme that catalyzes the reaction of FMNH2 to DMB used in the AdoCbl synthesis pathway in <i>E. coli</i>. Also used in <a href="https://parts.igem.org/Part:BBa_K4604020">BBa_K4604020</a> , a more detailed characterization is uploaded there. Has a C-terminal his-tag.</html>
  
  

Latest revision as of 23:10, 11 October 2023


bluB mutated

This BioBrick is a mutated Version of BioBrick BBa_K4604005, the gene for the BluB enzyme that catalyzes the reaction of FMNH2 to DMB used in the AdoCbl synthesis pathway in E. coli. Also used in BBa_K4604020 , a more detailed characterization is uploaded there. Has a C-terminal his-tag.


Figure 1: Vitamin B12 production from precursors in E. coli. The BluB transforms the reduced form of flavin mononucleotide (FMNH2) to form 5,6-dimethylbenzimidazole (DMB) which then joins exogenously-provided cobinamide to create vitamin B12. Made with biorender.com



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]