Difference between revisions of "Part:BBa K4825047:Design"

 
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===References===
 
===References===
 +
1. Willer, M., Rainey, M., Pullen, T., and Stirling, C. J. (1999) The yeast CDC9 gene encodes both a nuclear and a mitochondrial form of DNA ligase I. Curr. Biol. 9, 1085−1094.
 +
 +
2. Sarah K. Hammer, Yanfei Zhang, and José L. Avalos

Revision as of 09:07, 12 October 2023


CDC9-LEU1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1163
    Illegal EcoRI site found at 1627
    Illegal EcoRI site found at 2917
    Illegal EcoRI site found at 3706
    Illegal XbaI site found at 1404
    Illegal PstI site found at 2093
    Illegal PstI site found at 2891
    Illegal PstI site found at 3386
    Illegal PstI site found at 3671
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1163
    Illegal EcoRI site found at 1627
    Illegal EcoRI site found at 2917
    Illegal EcoRI site found at 3706
    Illegal PstI site found at 2093
    Illegal PstI site found at 2891
    Illegal PstI site found at 3386
    Illegal PstI site found at 3671
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1163
    Illegal EcoRI site found at 1627
    Illegal EcoRI site found at 2917
    Illegal EcoRI site found at 3706
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1163
    Illegal EcoRI site found at 1627
    Illegal EcoRI site found at 2917
    Illegal EcoRI site found at 3706
    Illegal XbaI site found at 1404
    Illegal PstI site found at 2093
    Illegal PstI site found at 2891
    Illegal PstI site found at 3386
    Illegal PstI site found at 3671
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1163
    Illegal EcoRI site found at 1627
    Illegal EcoRI site found at 2917
    Illegal EcoRI site found at 3706
    Illegal XbaI site found at 1404
    Illegal PstI site found at 2093
    Illegal PstI site found at 2891
    Illegal PstI site found at 3386
    Illegal PstI site found at 3671
    Illegal NgoMIV site found at 2434
    Illegal AgeI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 3896


Design Notes

The composite part appends the first 47 amino acids of CDC9 to the N-terminus of LEU1 in order to relocate and overexpress LEU1 in mitochondria.


Source

Saccharomyces cerevisiae

References

1. Willer, M., Rainey, M., Pullen, T., and Stirling, C. J. (1999) The yeast CDC9 gene encodes both a nuclear and a mitochondrial form of DNA ligase I. Curr. Biol. 9, 1085−1094.

2. Sarah K. Hammer, Yanfei Zhang, and José L. Avalos