Difference between revisions of "Part:BBa K4960014"
| Line 10: | Line 10: | ||
<!-- Add more about the biology of this part here--> | <!-- Add more about the biology of this part here--> | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
| − | Photorhabdus virulence cassette (PVC) is one group of extracellular Contractile injection systems (CISs) that are cell-puncturing nanodevices for targeted delivery of proteins .[1]Structurally similar to other CISs, PVCs are also macromolecular complexes containing a rigid tube structure housed in a contractile sheath, which is anchored to a baseplate and sharpened by a spike protein.[2](Figure1)<br> | + | Photorhabdus virulence cassette (PVC) is one group of extracellular Contractile injection systems (CISs) that are cell-puncturing nanodevices for targeted delivery of proteins .[1]Structurally similar to other CISs, PVCs are also macromolecular complexes containing a rigid tube structure housed in a contractile sheath, which is anchored to a baseplate and sharpened by a spike protein.[2]('''Figure1''')<br> |
<html> | <html> | ||
| Line 19: | Line 19: | ||
</html> | </html> | ||
| − | Figure 1. Schematic of the PVCpnf locus. It contains 16 structural and accessory genes , followed by two payload genes (Pdp1 and Pnf, in red) and four putative regulatory genes (in pink).<br> | + | '''Figure 1. Schematic of the PVCpnf locus. '''It contains 16 structural and accessory genes , followed by two payload genes (Pdp1 and Pnf, in red) and four putative regulatory genes (in pink).<br> |
<br> | <br> | ||
Pvc15 is present at very low levels in the PVC syringes sample and is absent in CISs like phage T4 or R-type pyocins.According to previous studies,it possibly functions as tape measure protein and ATPase. | Pvc15 is present at very low levels in the PVC syringes sample and is absent in CISs like phage T4 or R-type pyocins.According to previous studies,it possibly functions as tape measure protein and ATPase. | ||
Revision as of 03:38, 12 October 2023
pvc15
Pvc15 functions as tape measure protein and ATPase.
Profile
Name: PVC15
Base Pairs: 2097bp
Origin: Photorhabdus
Properties: Pvc15 functions as tape measure protein and ATPase.
Usage and Biology
Photorhabdus virulence cassette (PVC) is one group of extracellular Contractile injection systems (CISs) that are cell-puncturing nanodevices for targeted delivery of proteins .[1]Structurally similar to other CISs, PVCs are also macromolecular complexes containing a rigid tube structure housed in a contractile sheath, which is anchored to a baseplate and sharpened by a spike protein.[2](Figure1)
Pvc15 is present at very low levels in the PVC syringes sample and is absent in CISs like phage T4 or R-type pyocins.According to previous studies,it possibly functions as tape measure protein and ATPase.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 87
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 618
Illegal NgoMIV site found at 757
Illegal AgeI site found at 1056 - 1000COMPATIBLE WITH RFC[1000]
References
[1]Kreitz J, Friedrich MJ, Guru A, Lash B, Saito M, Macrae RK, Zhang F. Programmable protein delivery with a bacterial contractile injection system. Nature. 2023 Apr;616(7956):357-364. doi: 10.1038/s41586-023-05870-7. Epub 2023 Mar 29.
[2]Jiang F, Li N, Wang X, Cheng J, Huang Y, Yang Y, Yang J, Cai B, Wang YP, Jin Q, Gao N. Cryo-EM Structure and Assembly of an Extracellular Contractile Injection System. Cell. 2019 Apr 4;177(2):370-383.e15. doi: 10.1016/j.cell.2019.02.020. Epub 2019 Mar 21.