Difference between revisions of "Part:BBa F1610"
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'''We use this part on iGEM2009 plate. While present on a gel after cutting the miniprep DNA extraction by EcoRI and SpeI, the restriction fragment appears to about 3000 bp and 800 bp . BBa_F1610 on iGEM2009 plate is therefore correct.'''--[[User:Koi|Koi]] 20 Oct 2009 (Tokyo_Tech) | '''We use this part on iGEM2009 plate. While present on a gel after cutting the miniprep DNA extraction by EcoRI and SpeI, the restriction fragment appears to about 3000 bp and 800 bp . BBa_F1610 on iGEM2009 plate is therefore correct.'''--[[User:Koi|Koi]] 20 Oct 2009 (Tokyo_Tech) | ||
| − | [[:Image:F1610_restriction2009.jpg|See gel | + | |
| + | [[:Image:F1610_restriction2009.jpg|See gel image]] | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 09:40, 20 October 2009
3OC6HSL Sender Device
This device accepts PoPS as input and produces the LuxI enzyme. This enzyme produces 3OC6HSL.
Notice
With a VF2-VR PCR length compraison, I found that it is empty on this plasmid sent in iGEM2007 plates. --marion 07:12, 22 March 2008 (EDT)
While present on a gel after running PCR on both 2007 and 2008 plates, the DNA appears to only be ~600bp long which conflicts with the part description. --robere 27 Jun 2008
See gel image
We use this part on iGEM2009 plate. While present on a gel after cutting the miniprep DNA extraction by EcoRI and SpeI, the restriction fragment appears to about 3000 bp and 800 bp . BBa_F1610 on iGEM2009 plate is therefore correct.--Koi 20 Oct 2009 (Tokyo_Tech)
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 655
- 23INCOMPATIBLE WITH RFC[23]Unknown
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]