Difference between revisions of "Part:BBa K4830008:Design"
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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The design of ngRNA includes the U6 promoter, spacer, guide RNA scaffold, and polyA tail. The unique ngRNA was designed based on SNP data.
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The design of pegRNA includes the U6 promoter, spacer, guide RNA scaffold, rttpbs and polyA tail. The unique pegRNA was designed based on SNP data.
  
 
===Source===
 
===Source===

Revision as of 20:54, 11 October 2023


POAG snp1-3 ngRNA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The design of pegRNA includes the U6 promoter, spacer, guide RNA scaffold, rttpbs and polyA tail. The unique pegRNA was designed based on SNP data.

Source

SNPs were retrieved from the paper cited in References.

References

Gharahkhani, P. et al. (2021) ‘Genome-wide meta-analysis identifies 127 open-angle glaucoma loci with consistent effect across ancestries’, Nature Communications, 12(1). doi:10.1038/s41467-020-20851-4.