Difference between revisions of "Part:BBa K4788006"

 
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Transforming cholesterol into 4-cholesten-3-one while producing short chain fatty acids.
 
Transforming cholesterol into 4-cholesten-3-one while producing short chain fatty acids.
  
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===Usage and Biology===
 
===Usage and Biology===
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It is derived from the combination of the IsmA gene and the BCoAT gene, which conforms to the part. It can directly degrade cholesterol while producing short chain fatty acids to indirectly regulate cholesterol.
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===Characterization===
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In order to characterize the corresponding components, we need to develop different characterization schemes for different genes. For the BCoAT gene, We use gas chromatography to examine the production of short chain fatty acids in strains containing the BCoAT gene to characterize the gene, and for the IsmA gene, we used the OPA method to detect cholesterol residue after cholesterol culture medium cultivation to characterize it.
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Meanwhile, as short chain fatty acids are also important cholesterol regulatory substances, we can also construct the IsmA-BSH-BCoAT triplet gene to achieve more complex cholesterol regulation. For more information on triplets, please refer to <html><a style="padding: 0px; margin: 0px;" href="https://parts.igem.org/Part:BBa_K4788007"> BBa_K4788007</a ></html>.
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In the characterization of the triad, as shown in Figure 1, we can see that the IsmA gene in the I-BC gene has been successfully characterized. As shown in Figure 2,We can also see the characterization of the BCoAT gene from its GC data.However, the characterization of BCoAT in the binary system is not significant
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<html>
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<figure style="text-align:center;">
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                <img style="max-width:700px;" src="https://static.igem.wiki/teams/4788/wiki/proof/cholesterol.jpg" alt="control">
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                <figcaption><b>Figure1:</b> a) Cholesterol residue in the culture medium after cultivation of engineering bacteria on basic cholesterol medium b) Percentage of cholesterol degradation in the culture medium after cultivation of engineering bacteria on basic cholesterol medium</figcaption>
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              </figure>
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</html>
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<html>
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<figure style="text-align:center;">
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                <img style="max-width:700px;" src="https://https://static.igem.wiki/teams/4788/wiki/proof/bcoat.jpg" alt="control">
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                <figcaption><b>Figure1:</b> GC detection of short chain fatty acid production by BCoAT containing strains</figcaption>
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              </figure>
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</html>
  
 
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Revision as of 03:02, 12 October 2023


IsmA-BCoAT

Transforming cholesterol into 4-cholesten-3-one while producing short chain fatty acids.

Usage and Biology

It is derived from the combination of the IsmA gene and the BCoAT gene, which conforms to the part. It can directly degrade cholesterol while producing short chain fatty acids to indirectly regulate cholesterol.

Characterization

In order to characterize the corresponding components, we need to develop different characterization schemes for different genes. For the BCoAT gene, We use gas chromatography to examine the production of short chain fatty acids in strains containing the BCoAT gene to characterize the gene, and for the IsmA gene, we used the OPA method to detect cholesterol residue after cholesterol culture medium cultivation to characterize it. Meanwhile, as short chain fatty acids are also important cholesterol regulatory substances, we can also construct the IsmA-BSH-BCoAT triplet gene to achieve more complex cholesterol regulation. For more information on triplets, please refer to BBa_K4788007. In the characterization of the triad, as shown in Figure 1, we can see that the IsmA gene in the I-BC gene has been successfully characterized. As shown in Figure 2,We can also see the characterization of the BCoAT gene from its GC data.However, the characterization of BCoAT in the binary system is not significant

control
Figure1: a) Cholesterol residue in the culture medium after cultivation of engineering bacteria on basic cholesterol medium b) Percentage of cholesterol degradation in the culture medium after cultivation of engineering bacteria on basic cholesterol medium
control
Figure1: GC detection of short chain fatty acid production by BCoAT containing strains

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1861
    Illegal AgeI site found at 58
  • 1000
    COMPATIBLE WITH RFC[1000]