Difference between revisions of "Part:BBa K176012"

Revision as of 10:23, 21 October 2009

pCon 0.70(J23101)->RBS+GFP+T

pCon 0.70(J23101)+I13504

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 705

MEASUREMENT

We measured five constitutive promoter family member ( J23100, J23101, J23103, J23107, J23109, J23115, )strength by ligated each with a 
GFP gene ( I13504) , and then moniter the OD600 and GFP expression.
K176011: J23100 + GFP ( I13504 )
K176012: J23101 + GFP ( I13504)
K176013: K176009( J23107) + GFP ( I13504)
K176014: K176008( J23115) + GFP ( I13504)
K176015: J23109 + GFP ( I13504)
K176016: J23103 + GFP ( I13504)

Model

To see the details of the model, please click [http://2009.igem.org/Team:USTC/Modeling/Model-1 here]

data

J23101+GFP

COLONY NUMBER	T(min)	OD600	FLU(arbitrary)	ST.FLU(ustc_st.1)	FLU/OD600(arbitrary)	ST.FLU/OD600(ustc_st.1)
Ⅰ	0	0.117666667	142.91	571.2515489	1214.532578	4854.829028
Ⅰ	30	0.119666667	152.6266667	610.0918042	1275.431755	5098.260203
Ⅰ	60	0.143666667	181.65	726.1062478	1264.385151	5054.103813
Ⅰ	90	0.174666667	206.08	823.7598433	1179.847328	4716.182309
Ⅰ	120	0.198	220.6666667	882.0668612	1114.478114	4454.883137
Ⅰ	150	0.245666667	242.8866667	970.8864639	988.6838535	3952.048021
Ⅰ	180	0.392	276.32	1104.52892	704.8979592	2817.675817
Ⅱ	0	0.083	115.0533333	459.9006009	1386.184739	5540.971095
Ⅱ	30	0.098333333	126.3533333	505.0698858	1284.949153	5136.303924
Ⅱ	60	0.112	144.0466667	575.795126	1286.130952	5141.027911
Ⅱ	90	0.129666667	188.7433333	754.4603003	1455.604113	5818.4599
Ⅱ	120	0.14	174.4166667	697.1925757	1245.833333	4979.946969
Ⅱ	150	0.169333333	184.28	736.619099	1088.267717	4350.112789
Ⅱ	180	0.251333333	232.6733333	930.0608919	925.7559682	3700.507528

For the details of the definition of ST.FLU and the unit ustc_st1, please click here.

plot

protocol

1. Streak a plate of the strain which contain one of the parts listed in pSB1A3 .

2. Inoculate two 3ml cultures of supplemented M9 Medium and antibiotic( Ampicillin 0.1mg/ml) with single colony from the plate.

3. Cultures were grown in test tubes（BIO BASIC INC.12ml Polypropylene Round-bottom Culture Tubes With Graduations And Dual Cap Cat.No:TD444） for 16hrs at 37℃ with shaking at 200rpm.

4. Cultures were diluted 1:100 into 3ml fresh medium and grown for 3hrs.

5. Measure the fluorescence(SHIMDZU SPECTROFLUOROPHOTOMETER RF-5301PC, 250ul quartz cell path length 10mm,501 nm excitation,514 nm emission,1.5nm slit width) and absorbance (HITACHI UV-VIS spectrophotometer U-2810 ,200ul quartz cell,path length 10mm,600nm,1.5 nm slit width) every 30 minutes in the next 4hrs.

@@ Line 20: / Line 20: @@
   K176015: J23109 + GFP ( I13504)
   K176016: J23103 + GFP ( I13504)
+==Model==
+To see the details of the model, please click [http://2009.igem.org/Team:USTC/Modeling/Model-1 here]
 ===data===
 J23101+GFP